Structural changes of the rabbit retina after 14-days tamponade of the vitreal cavity with perfluoro-organic compounds
DOI:
https://doi.org/10.31288/oftalmolzh201555257Keywords:
light optical and ultrastructural changes, retina, perfluoro-organic compounds (perfluorocarbonliquid), light and heavy silicone oilAbstract
Introduction. Perfluoro-organic compounds (PFOC) have valuable qualities for vitreoretinal surgery and their application for short-term tamponade could expand indications for preoperative treatment and improve its results. However, there is no unambiguous opinion concerning the mechanical action of PFOC on ultrastructural structure of retina.
The purpose of the study: To study the influence of 14-days PFOC tamponade on the structure of the rabbit in experiment; to compare the action of PFOC, saline solution, light and heavy silicone oil in dynamics after the tamponade completion.
Material and methods. Light and electro- microscopic study was carried out on 36 rabbits (72 eyes). All animals underwent back closed subtotal vitrectomy followed by 14-days PFOC tamponade (right eye), light and heavy silicone and saline solution (left eye). The study was conducted 7, 14 and 30 days after the completion of 14-days tamponade.
Results. PFOC, light and heavy silicone cause similar changes in the ultrastructure of the studied retina elements. These are mainly hydropic changes of smooth endoplasmic reticulum and mitochondria of the retinal pigment epithelium (RPE) cells, mitochondria of inner segments of photoreceptor cells, ganglion cells and Muller cells. At the same time, these cells also show the signs of compensatory-restorative processes, which is reflected in increasing of protein synthesis and energy producing activity that contributes to normalization of the revealed damage of ultrastructure. It should be noted that PFOC and light silicone have particularly similar effect on the retina, while heavy silicone causes more pronounced reaction at subcellular level. Introduction of the saline solution during the first periods of observation leads to the light reactive hydropic changes, mainly in PRE cells.
Conclusion. Revealed hydropic changes in the retina after the completion of 14-days tamponade with the used substances are classified as reactive, not damaging changes and are reversible. By the end of the periods of observation the ultrastructure of the retina practically restores. Therefore PFOC can be considered as an alternative for a short-term tamponade.
References
1.Takhchidi KP, Kostin OA. [Specifics of the surgery of tractional retinal detachment at proliferative diabetic retinopathy]. Perfluoro-organic compounds in Biology and Medicine. Sb Nauch Tr. Pushchino. 1999:192-194. Russian.
2.Shkvorchenko DO. [Complex surgical treatment of the retinal detachment, complicated by giant gaps and separation from dentate line with application of liquid perfluoro-organic compounds]. Dis…PhD in Med. Sci. 14.00.08. Moscow. 1995: 132. Russian.
3.Shkvorchenko DO, Kashtan OV, Makarov KN, Ronkina TI. [Experimental clinical application of vitreopress for the short-term postoperative tamponade in vitreoretinal surgery]. Perfluoro-organic compounds in Biology and Medicine. Sb Nauch Tr. Pushchino. 1999: 186-192. Russian.
4.Chang S, Sparrow JR, Iwamoto T, Gershbein A, Ross R, Ortiz R. Experimental studies of tolerance to intravitreal perfluoronoctane liquid. Retina. 1991; 11: 367-374. https://doi.org/10.1097/00006982-199110000-00001
5.Clark LC Jr, Gollan F. Survival of mammals breathing organic liquids equilibrated with oxygen at atmospheric pressure. Science. 1966; 152: 1755-1756.https://doi.org/10.1126/science.152.3730.1755
6.Devin F, Jourdan T, Saracco JB, Lucciani A. Experimental tolerability of perfluorodecalin in prolonged intraocular tamponade. J Fr Ophtalmol. 1995; 18: 268-274.
7.Flores-Aguilar M, Munguia D, Loeb E, Crapotta JA, Vuong C, Shakiba S, Bergeron-Lynn G, Wiley CA, Weers J, Freeman WR. Intraocular tolerance of perfluorooctylbromide (perflubron). Retina. 1995; 15: 3-13.https://doi.org/10.1097/00006982-199515010-00002
8.Haidt SJ, Clark LC, Ginsberg J. Liquid perfluorocarbon replacement of the eye. Invest. Ophthalmol.VisSci. 1982; 22: 233.
9.Norman H J. Requirements of bioethics of the Helsinki declaration about ethical regulation of medical researches. Chronicle of the World Organization of Healthcare. 1985; 39 (3): 3-9
10.Orzalesi N, Migliavacca L, Bottoni F, Miglior S. Experimental short-term tolerance to perfluorodecalin in the rabbit eye: a histopathological study. Curr Eye Res. 1998; 17: 828-835.https://doi.org/10.1080/02713689808951265
11.Reynoldes ES. The use of lead citrate at high pH an electronopaque stain in electron microscopy. I. Cell Biol. 17: 208-212.https://doi.org/10.1083/jcb.17.1.208
12.Sirimaharaj M, Balachandran C, Chan WC, Hunyor AP, Chang AA, Gregory-Roberts J, Hunyor AB, Playfair TJ. [Vitrectomy with short term postoperative tamponade using perfluorocarbonliquid for giant retinal tears]. Br J Ophthalmol. 2005; 89: 1176-1179.https://doi.org/10.1136/bjo.2004.065409
13.Terauchi H, Okinami S, Kozaki Z, Tanihara H, Nagata M, Segawa Y. Experimental study on the effects of a replacement of the vitreous body with perfluorotributylamine on the rabbit eye. Nihon Ganka Gakkai Zasshi. 1989; 93: 294-301.
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