Ultrastructural changes in the rabbit chorioretinal complex following 577-nm laser photocoagulation
DOI:
https://doi.org/10.31288/oftalmolzh201745559Keywords:
chorioretinal complex, laser radiation, ultrastructural changes, rabbit eyeAbstract
Background: There is no unanimously adopted approach to titrating laser parameters in subthreshold retinal laser photocoagulation without ophthalmoscopically visible fundus changes.
Purpose: To investigate the ultrastructural changes in the rabbit chorioretinal complex following 577-nm conventional, selective or micropulse retinal laser photocoagulation (RLPC).
Materials and Methods: Four rabbits (8 eyes) were involved in the experimental study and underwent the RLPC modes with the 577-nm system (Supra 577). Rabbits (n = 2/time point) were euthanized at postlaser days 1 and 14. Ultra-thin sections of their ocular tissue specimens were subjected to electron microscopy. In addition, 2 intact rabbits (4 eyes) were used as controls for comparison.
Results: Conventional RLPC resulted in damage to RPE cells, all photoreceptor compartments and choriocapillaries; selective RLPC resulted in damage to RPE cells and photoreceptor outer and inner segments; and micropulse RLPC with the power setting adjusted to 50% threshold resulted in damage mostly to apical aspects of RPE cells and photoreceptor outer segments.
Conclusion: 577-nm micropulse RLPC with the power setting adjusted to 50% threshold is a more photoreceptor- and choriocapillaris-sparing approach compared to conventional and selective RLPC, and can be used in clinical practice as a laser treatment approach with the least invasive effect on the chorioretinal complex.
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