Влияние дефицита слезной жидкости на развитие эндотоксин-индуцируемого кератита

T.B. Gaydamaka; S.Ya. Rafalyuk

doi:10.31288/oftalmolzh201544448

Authors

T.B. Gaydamaka SI "The Filatov Institute of Eye Diseases and Tissue Therapy of the NAMS of Ukraine"; Odesa (Ukraine)
S.Ya. Rafalyuk Danylo Halytsky Lviv National Medical University; Lviv (Ukraine)

DOI:

https://doi.org/10.31288/oftalmolzh201544448

Keywords:

endotoxin-induced keratitis, dry eye syndrome, animal models, tear deficiency, corneal inflammation, Draize criteria

Abstract

Background:In recent years, the problem of tear production disorders and dry eye syndrome (DES) has become increasingly important because of significant worldwide prevalence of DES.

Purpose: To investigate the effect of decreased tear production on the course of endotoxin-induced keratitis in rabbits.

Materials and Methods: Animals were divided into three groups: group 1 (controls; 7 rabbits), group 2 (keratitis-only group; 9 rabbits, 18 eyes), and group 3 (keratitis + dry eye model group; 10 rabbits; 20 eyes). The state of the cornea was scored by Draize criteria (the degree of corneal opacity, degree of corneal edema, degree of corneal infiltration and corneal fluorescein staining area) at preselected intervals (24, 48 and 72 hours) postinfection.

Results: It should be noted that the analysis of clinical scores of corneal inflammation in the models of intrastromal endotoxin-induced keratitis in intact animals and in those with experimental dry eye showed that the clinical manifestations in the latter were more pronounced.

Conclusion: Tear deficiency significantly contributes to corneal inflammation in animal models of keratitis. Within the observation period, mean corneal edema, inflammatory infiltration and fluorescein staining scores in the (dry eye + keratitis) rabbit model were 21%, 21% and 17%, respectively higher than those in endotoxin-induced keratitis in animals without dry eye.

References

1. Anina EI. [Prevalence of corneal disorders in Ukrainian population]. In: [Proceedings of the 2nd International Conference of the Black Sea Ophthalmological Society]; 2004 Sep 8-10; Odessa. p.14. Russian.

2. Albietz JM. Prevalence of dry eye subtypes in clinical optometry practice. Optom Vis Sci. 2000 Jul;77(7):357-63.https://doi.org/10.1002/j.1538-9235.2000.tb01116.x

3. Gaydamaka TB, Rafalyuk SYa. [Effect of endotoxin-induced keratitis on the reduction potential of glutathione in the cornea of animals with experimental dry eye syndrome]. Oftalmol Zh. 2014;(6):54-7. Russian.

4. Zhaboedov GD, Kireev VV. [Dry eye syndrome: Clinical picture, diagnosis and treatment. Guidelines for medical students and practitioners]. Kyiv, 2006. 24 p. Russian.

5. Somov EE. [Tear dysfunction syndrome: anatomic and physiological basis, clinical picture and treatment]. St. Petersburg: Chelovek; 2011. 160 p. Russian.

6. Trinkaus-Randall V, Leibowitz HM, Ryan WJ, Kupferman A. Quantification of stromal destruction in the inflamed cornea. Invest Ophthalmol Vis Sci. 1991 Mar;32(3):603-9.

7. Brzheski? VV, Astakhov IuS, Kuznetsova NIu. [Tear system disorders: A manual for ophthalmic practitioners]. 2nd rev. ed. St. Petersburg: N-L; 2009. 108 p. Russian.

8. Barabino S, Chen W, Dana MR. Tear film and ocular surface tests in animal models of dry eye: uses and limitations. Exp Eye Res. 2004;79:613-21.https://doi.org/10.1016/j.exer.2004.07.002

9. Brewitt H, Sistani F. Dry eye disease: The scale of the problem. Surv Ophthalmol. 2001;45(Suppl. 2):S199-202.https://doi.org/10.1016/S0039-6257(00)00202-2

10. Kim JR, Oh TH, Kim HS. Effects of benzalkonium chloride on the ocular surface of the rabbit. Jpn J Ophthalmol. 2011 May;55(3):283-93.https://doi.org/10.1007/s10384-011-0008-4

11. Trocme S, Hwang LJ, Bean GW, Sultan MB. The role of benzalkonium chloride in the occurrence of punctate keratitis: a meta-analysis of randomized, controlled clinical trials.Ann Pharmacother. 2010 Dec;44(12):1914-21.https://doi.org/10.1345/aph.1P268

12. Petrunia AM. [Pathogenetic features of keratitis in acute conjunctivitis]. [Cand. Sc. (Med) Thesis Abstract]. Odessa: Filatov Eye Disease and Tissue Therapy Institute;2013. 19 p.Russian.

13. Baudouin C. The pathology of dry eye. Surv Ophthalmol. 2001;45(Suppl. 2):S211-20.https://doi.org/10.1016/S0039-6257(00)00200-9

14. Bourcier T, Thomas F, Borderie V et al. Bacterial keratitis: predisposing factors, clinical and microbiological review of 300 cases. BrJ Ophthalmol. 2003;87(7):834-8.https://doi.org/10.1136/bjo.87.7.834

15. Carlson E C, Drazba J, Yang X, Perez V L. Invest Ophthalmol Vis Sci. 2006; 47: 241-8.https://doi.org/10.1167/iovs.04-0741

16. Yuan X, Wilhelmus KR, Matoba AY et al. Pathogenesis and outcome of Paecilomyces keratitis. Am J Ophthalmol. 2009 Apr;147(4):691-696.https://doi.org/10.1016/j.ajo.2008.11.016

17. Senyshyn VI, Rafalyuk SYa. [Effect of ophthalmic preservative, benzalkonium chloride, on the mitochondrial enzymes of anterior eye tissues]. Oftalmol Zh. 2014;(4):37-40. Russian.

18. Schultz CL, Morck DW, McKay SG et al. Lipopolysaccharide induced acute red eye and corneal ulcers. Exp Eye Res. 1997 Jan;64(1):3-9.https://doi.org/10.1006/exer.1996.0190

19. Schultz CL, Buret AG, Olson ME et al. Lipopolysaccharide entry in the damaged cornea and specific uptake by polymorphonuclear neutrophils. Infect Immun. 2000 Mar;68(3):1731-4.https://doi.org/10.1128/IAI.68.3.1731-1734.2000

20. Lin Z, Liu X, Zhou T et al. A mouse dry eye model induced by topical administration of benzalkonium chloride. Mol Vis. 2011; Jan 25;17:257-64.

21. Xiong C, Chen D, Liu J et al. A rabbit dry eye model induced by topical medication of a preservative benzalkonium chloride. Invest Ophthalmol Vis Sci. 2008 May;49(5):1850-6.https://doi.org/10.1167/iovs.07-0720

22. Nasledov AD. [SPSS. Computer data analysis in psychology and social science]. St. Petersburg: Piter; 2005. 416 p. Russian.