Нейродегенеративні зміни сітківки щурів з хронічною формою епілептичного синдрому за умови застосування ніацин-оксіетилідендифосфонатогерманату (МІГУ-4)

N. Al-Nadawi; V.I. Kresyun

doi:10.31288/oftalmolzh202322630

Authors

N. Al-Nadawi Odesa National Medical University https://orcid.org/0000-0003-0976-5897
V.I. Kresyun Odesa National Medical University https://orcid.org/0000-0003-0976-5897

DOI:

https://doi.org/10.31288/oftalmolzh202322630

Keywords:

pentylenetetrazol kindling, chronic epileptic syndrome, retinopathy, niacin-oxy-ethylidene-phosphonate germinate (MIGU-4), neuroprotection

Abstract

Background: Epilepsy is accompanied by neurodegenerative changes, particularly those in the retina. Elucidation of the mechanisms of retinal alterations in a model of epileptic syndrome may allow for the development of new approaches to pharmacological vision correction.

Purpose: To assess the morphological characteristics of the retina, particularly treated with niacin oxy-ethylidene phosphonate germinate (MIGU-4), in the pentylenetetrazole (PTZ)-induced kindling model of chronic epileptic syndrome.

Material and Methods: PTZ was administered in Wistar male rats intraperitoneally (i.p.) at a daily dose of 35.0 mg/kg for 21 days. Rats with fully-developed generalized seizures were treated with MIGU-4 ip at a daily dose of 5.0 mg/kg or 25.0 mg/kg for 28 days. Thereafter, the animals were euthanized, their globes were enucleated, and retinal sections were prepared and stained with hematoxylin and eosin for morphological examination.

Results: Cell density in the retinal ganglion cell layer was 2.14 times lower, and in the inner nuclear layer and outer nuclear layer, 41.0% and 19.0%, respectively, lower for the rats with fully developed kindled seizures than for controls (р < 0.05). In the presence of treatment with MIGU-4 ip at a daily dose of 25.0 mg/kg, cell density in the retinal ganglion cell layer and in the inner nuclear layer was 38% and 30.5%, respectively, higher than for controls (р < 0.05). There was no significant difference in the cell density in the outer nuclear layer between rats treated with MIGU-4 ip at a daily dose of 5 mg/kg or 25.0 mg/kg and controls (р > 0.05).

Conclusion: The PTZ-induced kindling model of chronic epileptic syndrome is accompanied by degenerative changes in the eye. A course of treatment with MIGU-4 causes neuroptotective effects in a model of PTZ-induced retinopathy.

Author Biography

N. Al-Nadawi, Odesa National Medical University

Assistant

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