Визначення кореляційної залежності між ступенем помутніння кришталика, загальною антиоксидантною активністю та продуктами пероксидації у слізній рідині хворих при катаракті та супутньому бактеріальному кератиті

V. Ia. Usov; Tarik  Abou Tarboush; S. G. Kolomiichuk

doi:10.31288/oftalmolzh202531519

Authors

V. Ia. Usov Petro Mohyla Black Sea National University, Mykolaiv, Ukraine
Abou Tarboush SI «The Filatov Institute of Eye Diseases and Tissue Therapy of the NAMS of Ukraine»
S. G. Kolomiichuk SI «The Filatov Institute of Eye Diseases and Tissue Therapy of the NAMS of Ukraine»

DOI:

https://doi.org/10.31288/oftalmolzh202531519

Keywords:

cataract, keratitis, lipid peroxidation, total antioxidant activity, antioxidant enzymes, methyl-ethyl pyridinol hydrochloride, lens, cornea, tear fluid

Abstract

Purpose: To determine correlations between the grade of lens opacity and tear levels of total antioxidant activity (TAA) and lipid peroxidation (LPO) products in patients having both cataract and bacterial keratitis treated versus not treated with methyl-ethyl pyridinol hydrochloride (MH).

Methods: Patients of the study group had both senile cataract and bacterial stromal keratitis; they received MH (nine four-week cycles of daily treatment with MH separated by four-week breaks) as an adjunct to the conventional antibacterial treatment. Patients that received the conventional antibacterial treatment only were used as controls. The development of senile cataract in patients with keratitis was followed up for 18 months. Severity of lens opacity was assessed by slit lamp biomicroscopy and scored on a scale of 0 to 5. Tear levels of TAA, malondialdehyde (MDA) and diene conjugate (DC) were determined in patients with keratitis only and those having both keratitis and cataract. Spearman’s rank correlation was used to determine associations between the grade of lens opacity and tear levels of metabolic characteristics.

Results: Correlations between the grade of lens opacity and tear levels of TAA, MDA and DC were higher for patients having both cataract and keratitis and treated with MH than for patients with keratitis only. There were correlations of the grade of lens opacity with tear levels of TAA, MDA and DC (R=-0.85, R=0.80 and R=0.65, respectively) and of the tear level of TAA with tear levels of MDA and DC (R=-0.84 and R=-0.72, respectively) for patients having both cataract and bacterial keratitis and treated with MH.

Conclusion: Our findings of apparent correlations of the grade of lens opacity with the parameters of the oxidative-antioxidatiove system in the tear fluid of patients indicate a pathogenetic role of bacterial keratitis in the development of cataract. MH has antioxidative properties and may be used for correcting metabolic abnormalities and preventing structural and functional changes in the lens in cataractogenesis with corneal inflammation.

Author Biographies

V. Ia. Usov, Petro Mohyla Black Sea National University, Mykolaiv, Ukraine

Usov V. Ia, Dr Sc (Med), Prof., Petro Mohyla Black Sea National University, Mykolaiv (Ukraine)

Abou Tarboush , SI «The Filatov Institute of Eye Diseases and Tissue Therapy of the NAMS of Ukraine»

Tarik Abou Tarboush, Post-Graduate Student, SI “The Filatov Institute of Eye Diseases and Tissue Therapy of the National Academy of Medical Sciences of Ukraine”, Odesa (Ukraine)

S. G. Kolomiichuk, SI «The Filatov Institute of Eye Diseases and Tissue Therapy of the NAMS of Ukraine»

Kolomiichuk S. G, Researcher, Biochemistry Laboratory, SI “The Filatov Institute of Eye Diseases and Tissue Therapy of the National Academy of Medical Sciences of Ukraine”, Odesa (Ukraine)

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