Ultrastructural changes of the rabbits retina after threshold laser radiation with a wavelength of 532 nm, 577nm and 810 nm

Authors

  • N. E. Dumbrova State Institution The Filatov Institute of Eye Diseases and Tissue Therapy of the NAMS of Ukraine, Odessa, (Ukraine)
  • N. I. Molchanyuk State Institution The Filatov Institute of Eye Diseases and Tissue Therapy of the NAMS of Ukraine, Odessa, (Ukraine)
  • T. A. Romanova State Institution The Filatov Institute of Eye Diseases and Tissue Therapy of the NAMS of Ukraine, Odessa, (Ukraine)
  • N. M. Gavronskaya State Institution The Filatov Institute of Eye Diseases and Tissue Therapy of the NAMS of Ukraine, Odessa, (Ukraine)

DOI:

https://doi.org/10.31288/oftalmolzh201468994

Keywords:

ultrastructural changes of rabbit retinal of the outer and inner layers, threshold laser action

Abstract

In this article studied the ultrastructural changes of rabbit retinal neuroepithelial after exposure to lasers of different wavelengths. Laser photocoagulation remains the «gold» standard of care for retinal vascular disease. Under active study of the effects of laser energy at different wavelengths on the retina, the search for alterna¬tive methods of dosing of laser energy, as well as the search for optimal laser scars schemes applying to the affected area of the retina. The aim of the work was to study the effect of the threshold laser action on rabbit retinal ultrastructure of the outer and inner layers lasers generating radiation with a wavelength of 532 nm, 577 nm and 810 nm by electron microscopy. Work performed on 5 adult rabbits (10 eyes) Chinchilla, age 6 or 7 months, weighing 2.5—3 kg. Laser scars apply in the lower segments of the eyes, which were divided arbitrarily into two parts to perform the confluent and not confluent laser photocoagulation, using laser radiation of different wavelengths. Laser effect on the rabbit retina was performed in millipulse mode laser used laser power threshold effects. Power laser exposure was adjusted until a visible white scar 1—2 degrees. Control was retina intact rabbits. Found that in the midst of the threshold laser action occurs irreversible destruction of photoreceptor cells after all types of laser exposure. Least damaging effect on the bipolar ganglion cells and Muller has a laser wavelength of 810 nm as compared with the laser wavelength 577 nm and 532 nm when used in a threshold mode.

References

1.Branch Vein Occlusion Study Group. Argon laser photocoagulation for macular edema in branch vein occlusion. Am J Ophthalmol. 1984; 98: 271- 82.https://doi.org/10.1016/0002-9394(84)90316-7

2.Early Treatment Diabetic Retinopathy Study Research Group. Focal photocoagulation treatment of diabetic macular edema (Relationship of treatment effect to fluorescein angiographic and other retinal characteristics at baseline: ETDRS report no 19). Arch Ophthalmol. 1995;5113:1144- 55.https://doi.org/10.1001/archopht.1995.01100090070025

3.Guyer DR, D'Amico DJ, Smith CW. Subretinal fibrosis af¬ter laser photocoagulation for diabetic macular edema. Am J Ophthalmol. 1992;113:652- 6.https://doi.org/10.1016/S0002-9394(14)74789-0

4.Han DP, Mieler WF, Burton TC. Submacular fibrosis after photocoagulation for diabetic macular edema. Am J Ophthalmol. 1992;113:513-21.https://doi.org/10.1016/S0002-9394(14)74722-1

5.Ishiko S, Ogasawara H, Yoshida A et al. The use of scanning laser ophthalmoscope microperimetry to detect visual impairment caused by macular photocoagulation. Oph¬thalmic Surg Lasers. 1998;29:95- 8.https://doi.org/10.3928/1542-8877-19980201-03

6.Lewen RM. Subretinal neovascularization complicating laser photocoagulation of diabetic maculopathy. Ophthalmic Surg. 1988;19:734- 7.https://doi.org/10.3928/1542-8877-19881001-11

7.Lewis H, Schachat AP, Haimann MH et al. Choroidal neo-vascularization after laser photocoagulation for diabetic macular edema. Ophthalmology. 1990;97:503- 10.https://doi.org/10.1016/S0161-6420(90)32574-5

8.Morgan CM, Schatz H. Atrophic creep of the retinal pigment epithelium after focal macular photocoagulation.Ophthalmology. 1989;96:96-103.https://doi.org/10.1016/S0161-6420(89)32924-1

9.Schatz H, Madeira D, McDonald HR, Johnson et al. Progressive enlargement of laser scars following grid laser photocoagulation for diffuse diabetic macular edema. Arch Ophthalmol. 1991;109:1549- 51.https://doi.org/10.1001/archopht.1991.01080110085041

10.Sinclair SH, Alaniz R, Presti P. Laser treatment of diabetic macular edema: comparison of ETDRS-level treatment with threshold-level treatment by using high-contrast discriminant central visual field testing. Semin Ophthalmol. 1999;14: 214-22.https://doi.org/10.3109/08820539909069540

Published

2014-12-28

Issue

Section

Experimental Studies

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