Effect of sodium hyaluronate on pathochemical indicators in the tissues of the anterior eye after a preservative benzalkonium chloride application

Authors

  • V. Senishin Lvov Regional Hospital; (Ukraine)

DOI:

https://doi.org/10.31288/oftalmolzh201455360

Keywords:

the anterior eye, benzalkonium chloride, sodium hyaluronate

Abstract

Introduction. Importance of work is determined by clarification of the sodium hyaluronate effect on the pathochemical anterior eye tissue changes of animals treated with the instillation of preservative benzalkonium chloride (BAC).

Purpose. To study the effect of hyaluronate on the pathochemical indicators in the tissues of the anterior eye after application of the preservative benzalkonium chloride.

Methods. 22 rabbits were used for the experiment. All laboratory animals were divided into 2 groups: Group 1 — a control group (12 animals), the animals received the instillation of BAC, Group 2 — an experimental one (10 animals), the animals received the instillation of BAC and sodium hyaluronate. The activity of cytoplasmic, mitochondrial and lysosomal enzymes as well as reduced and oxidized glutathione were determined in the extracts obtained from the cornea, conjunctiva homogenates and lacrimal fluid.

Results. It was possible to weaken the negative effect of preservative BAC eye drops on the metabolic processes in the tissues of the anterior eye with hyaluronate. En­zyme activity was reduced under the influence of BAC, and the use of sodium hy-aluronate instillation restored enzyme activity: lactate dehydrogenase — 24.9 %, glucose-6phosphate dehydrogenase — 22.1 %, malate dehydrogenase — 23.9 %,glutamate dehydrogenase - 23.5 %, succinate dehydrogenase - 20.5 %, cyto-chrome oxidase - 27.3 %. Conclusions. Sodium hyaluronate significantly prevents the breakdown of enzy- matic activity caused by the preservative BAC in the tissues of the anterior eye.

References

1. Гайдамака Т. Б., Сенишин В. И. Влияние бензалкония хлорида на активность окислительно-восстановителных ферментов в тканях переднего отдела глаза // Офтальмол. журн. - 2013. - № 5. - С. 62- 67. https://doi.org/10.31288/oftalmolzh201356772

2. Гайдамака Т. Б., Сенишин В. И. Влияние консервантов глазных капель на восстановительный потенциал глутатиона в тканях переднего отдела глаза // Офталь-мол. журн. - 2012. - № 6. - С. 96- 100. http://doi.org/10.31288/oftalmolzh2012692100

3. Иванова Н. В. Консерванты в топической терапии глазных заболеваний: преимущества и недостатки // Офтальмология. Вост. Европа. - 2013. - Т.1. -№ 16. - С.2- 7.

4. Наследов A. SPSS компьютерный анализ данных в психологии и социальных науках / Наследов А. - Спб.: Питер, 2005. - 416 с.

5. Новые методы биохимического анализа. // Изд. Ле­нинградского универ. - 1991. - 395 с.

6. Ammar D. A., Noecker R. J., Kahook M. Y. Effects of benzalkonium chloride- preserved, polyquad-preserved, and sofzia-preserved topical glaucoma medications on human ocular epithelial cells // Adv. Ther. - 2010. - Vol. 27. -P. 1-9. https://doi.org/10.1007/s12325-010-0070-1

7. Ayaki M., Yaguchi S., Iwasawa A. Cytotoxicity of ophthalmic solution with and without preservatives to human corneal endothelial cells, epithelial cells and conjuncti­val epithelial cells // Clin. Exp. Ophthalmol. - 2008. - Vol. 36. - P. 553-559. https://doi.org/10.1111/j.1442-9071.2008.01803.x

8. Bark W. H., Tahir M. Effects of topical benzalkonium chloride on corneal epithelium // Biomedica. - 2007. - Vol. 23. - P. 65-70.

9. Bergmeyer H. V. Metoden der enzymatischen Analyse / Herausgegeben von H. U. Bergmeyer. - Berlin. - 1986. -2220 p.

10. Hopes M., Broadway D. C. Preservative- free Treatment in Glaucoma Is a Sensible and Realistic Aim for the Future // Europ. Ophthalmic. Review. - 2010. - Vol. 4. - P.23- 28. https://doi.org/10.17925/EOR.2010.04.01.23

11. Hughes E. H., Pretorius M., Eleftheriadis H. Long- term recovery of the human corneal endothelium after toxic injury by benzalkonium chloride // Br. J. Ophthalmol. - 2007. - Vol. 91. - P. 1460- 1463. https://doi.org/10.1136/bjo.2006.109439

12. Kahook M. Y., Noecker R. J. Comparison of corneal and conjunctival changes after dosing of travoprost preserved with sofZia, latanoprost with 0,02 % benzalkonium chlo­ride, and preservative- free artifical tears // Cornea. - 2008. - Vol. 27. - P. 339-343. https://doi.org/10.1097/ICO.0b013e31815cf651

13. Kim J. R., Oh T. H., Kim H. S. Effect of benzalkonium chloride on the ocular surface of the rabbit // Jpn. J. Ophthalmol. - 2011. - Vol. 55. - P. 283- 293. https://doi.org/10.1007/s10384-011-0008-4

14. Kozobolis V. P., Detorakis E. T., Maskaleris G. Corneal sensitivity changes following the instillation of latanoprost, bitamoprost, and travoprost eyedrops // Am. J. Ophthal-mol. - 2005. - Vol. 139. - P. 742-743. https://doi.org/10.1016/j.ajo.2004.10.021

15. Liang H., Baudouin C., Pauly A. Conjunctival and corneal reactions in rabbits following short- and repeated exposure to preservative- free tafluprost, commercially available latanoprost and 0,02 % benzalkonium chloride // Br. J. Ophthalmol. - 2008. - Vol. 92. - P. 1275- 1282. https://doi.org/10.1136/bjo.2008.138768

16. Lin Z., Liu X., Zhou T. A mouse dry eye model induced by topical administration of benzalkonium chloride // Mol. Vis. - 2011. - Vol. 17. - P. 257- 264.

17. McCarey B., Edelgauser H. In Vivo corneal epithelial permeability following treatment with prostaglandin analogues with or without benzalkonium chloride // J. Ocul.Pharm. Ther. - 2007. - Vol. 23. - P. 445-447. https://doi.org/10.1089/jop.2007.0024

18. Trocme S., Hwang L- J., Bean G. W. The role of benzalkonium chloride in the occurrence of punctate keratitis: a meta- analysis of randomized, controlled clinical trials //Ann. Pharmacother. - 2010. - Vol. 44. - P. 1914-1921. https://doi.org/10.1345/aph.1P268

19. Whitson J. T., Cavanagh H. D., Lakshman N. Assessment of corneal epithelial integrity after acute exposure to ocular hypotensive agents preserved with and without benzalkonium chloride // Adv. Ther. - 2006. - Vol. 23. - P. 663­671. https://doi.org/10.1007/BF02850305

20. Wilson F. M. Adverse external ocular effects of topical ophthalmic therapy: an epidemiologic, laboratory, and clinical study // Tr. Am. Ophth. Soc. - 1983. - Vol. 19. - P. 854­858.

21. Xiong C., Chen D., Liu J. A rabbit dry eye model induced by topical medication of a preservative benzalkonium chloride // Invest. Ophthalmol. Vis. Sci. - 2008. - Vol. 49. - P. 1850-1856. https://doi.org/10.1167/iovs.07-0720

Published

2014-10-30

Issue

No. 5 (2014): Journal of Ophthalmology (Ukraine)

Section

Experimental Studies

License

Copyright (c) 2014 В. И. Сенишин

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

This work is licensed under a Creative Commons Attribution 4.0 International (CC BY 4.0) that allows users to read, download, copy, distribute, print, search, or link to the full texts of the articles, or use them for any other lawful purpose, without asking prior permission from the publisher or the author as long as they cite the source.

COPYRIGHT NOTICE

Authors who publish in this journal agree to the following terms:

  1. Authors hold copyright immediately after publication of their works and retain publishing rights without any restrictions.
  2. The copyright commencement date complies the publication date of the issue, where the article is included in.

DEPOSIT POLICY

  1. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) during the editorial process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.
  2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work with an acknowledgement of its initial publication in this journal.
  3. Post-print (post-refereeing manuscript version) and publisher's PDF-version self-archiving is allowed.
  4. Archiving the pre-print (pre-refereeing manuscript version) not allowed.